摘要
Abstract
Objective To evaluate the effection of high mobility group box1protein on septic shock patients with the treatment of glutamine and to demonstrate the mechanism of the treatment of glutamine in patients with septic shock .Methods Seventy-two septic shock cases were selected in ICU of the hospital in May 2009 to July 2012 ,and then two groups were randomly divided ,just named control group and experimental group .After the traditional treatment ,such as fluid resuscitation ,antibiotic therapy and the use of breathing machine ,the control group received low-calorie parenteral nutrition and the experimental group received low-calorie parenteral nutrition combined with intravenous injection of glutamine .APACHEⅡscore ,the concentrations of serum glutamine and HMGB1 were detected at 4 different time points before treatment and the 1st ,3rd ,7th day after the treatment ,to analyze the effec-tion of the change of serum HMBD1 on septic shock patients with the treatment of glutamine .Results The serum glutamine and HMGB1 concentrations were detected with RP-HPLC and ELISA respectively .The result showed that with the intra-group com-parison ,APACHEⅡscores of both two groups after the treatment were lower than that before treatment ,and compare with the control ,the APACHEⅡscore of the experimental group was significantly lower .With disease duration increased ,the concentration of serum glutamine(409 .63 ± 158 .74)μmol/L in the control group was significantly decreased from 3 days after treatment ,that in the experimental group from 1 day (503 .92 ± 167 .66)μmol/L were gradually increased .the HMGB1 concentration in the control group did not appear change ,but that in the experimental group from 1 day (29 .24 ± 23 .91)ng/mL was decreased .Meanwhile ,the level of serum HMGB1[(34 .23 ± 23 .69)ng/mL ,(29 .24 ± 23 .91)ng/mL] concentration decreased synchronized with the changes in concentration of serum glutamine[(470 .05 ± 152 .40)μmol/L ,(503 .92 ± 167 .66)μmol/L] .Conclusion the application of Gluta-mine in treatment of septic shock existed positive significance for patients ,which can effectively increased the concentration of serum glutamine and reduced the concentration of HMGB1 ,to improve the prognosis of patients with septic shock .关键词
谷氨酰胺/脓毒症休克/高迁移率族蛋白B1/营养治疗Key words
glutamine/septic shock/high mobility group protein B1/nutrition therapy分类
医药卫生
