重庆医学Issue(23)：3031-3033,3036,4.DOI:10.3969/j.issn.1671-8348.2014.23.020
氢气对糖尿病大鼠心肌缺血／再灌注损伤心肌细胞凋亡及相关蛋白表达的影响
Effect of Hydrogen on ischemia/reperfusion-induced cardiocyte apoptosis in diabetic rats
摘要
Abstract
Objective To study the effect of Hydrogen on ischemia/reperfusion(I/R)-induced cardiocyte apoptosis and apoptosis related proteins expression in diabetic rats .Methods Diabetes mellitus was induced by intraperitoneal injection of streptozotocin (STZ) ,then feed 4 weeks before build ischemia/reperfusion model .60 SD male rats ,weight during 300-350 g ,were randomly divid-ed to six groups :non-diabetic sham-operated group (NS) ,non-diabetic I/R group(NI/R) ,non-diabetic hydrogen treated group (NH) ,diabetic sham-operated group(DS) ,diabetic I/R group(DI/R)and diabetic hydrogen treated group(DH) ,each group has 10 rats .The cardiac muscle I/R model was made by 30 min occlusion of the left anterior descending coronary artery and 2 h reperfu-sion .The rats in Hydrogen group were treated with 5 mL/kg hydrogen by intraperitoneal administration at the beginning of reper-fusion .The apoptosis index (AI) was calculated by TUNEL .The positive expressions of Bcl-2 ,Bax ,Caspase-3 in cardiomyocytes were respectively detected by immunohistochemistry .Results The apoptotic rates of cardiomyocytes and the positive expressions of Bcl-2 ,Bax ,Caspase-3 were significantly increased(P<0 .01) ,Compared with I/R group ,the apoptotic rates of cardiomyocytes in hydrogen treatment group were obviously decreased(P<0 .01) ,and the positive expressions of Bcl-2 were increased(P<0 .01) ,at the same time ,the positive expressions of Bax ,Caspase-3 were decreased(P<0 .01) .Conclusion Hydrogen inject by intraperitoneal method on myocardial ischemia-reperfusion injury of diabetic rats has a protective effect ;Its mechanism may be related to its inhibi-ting myocardial apoptosis by advanced the Bcl-2 protein expression and reduced the Bax 、Caspase-3 protein expression .关键词
氢气/糖尿病/心肌/缺血再灌注Key words
hydrogen/diabetes/myocardium/ischemia-reperfusion分类
医药卫生引用本文复制引用
卫然,林高翔,马菲菲,谭永星..氢气对糖尿病大鼠心肌缺血／再灌注损伤心肌细胞凋亡及相关蛋白表达的影响[J].重庆医学,2014,(23):3031-3033,3036,4.基金项目
广西医学科学实验中心开放课题（KFJJ2011-07）；广西卫生厅课题（Z2012402）。 （）
