重庆医学Issue(23)：3034-3036,3.DOI:10.3969/j.issn.1671-8348.2014.23.021
HEV ORF3 cDNA 序列分析及在 HepG2细胞的表达
DNA sequencing of HEV ORF3 cDNA and expression in HepG2 cell
摘要
Abstract
Objective To analysis the DNA sequence of HEV ORF3 cDNA and express in HepG2 cell .Methods Recombinant vector carrying HEV ORF3 cDNA was constructed and identified by DNA sequencing .Expression of recombinant vector in HepG2 cell was determined by immunofluorescence technique .Results The recombinant vector was constructed and expressed in HepG2 cell successfully .Conclusion The research laid experimental foundation for exploring the biological function of ORF3 protein in the pathogenesis of viral hepatitis E .关键词
戊型肝炎病毒/开放读码框架/表达载体Key words
hepatitis E virus(HEV)/open reading frame(ORF)/expression vector分类
医药卫生引用本文复制引用
赵振宇,温雯静,刘新胜,赵江波,刘晓东..HEV ORF3 cDNA 序列分析及在 HepG2细胞的表达[J].重庆医学,2014,(23):3034-3036,3.基金项目
河南省教育厅自然科学研究计划项目（2010C310005）。 （）
