重庆医学Issue(25)：3307-3310,4.DOI:10.3969/j.issn.1671-8348.2014.25.015
阻断p38MAPK信号通路对大鼠肝星状细胞活性及c-myc蛋白表达的影响
Effect of blocking p38MAPK signal pathway on activity of rat hepatic stellate cells and c-myc protein expression
摘要
Abstract
Objective To study the effect of p38MAPK on the activity and c-myc protein expression in rat acetaldehyde-induced hepatic stellate cell(HSC),and to investigate the alcoholic liver fibrosis related mechanism.Methods The different concentrations of SB203580 as the p38 specific blocker was adopted to conduct the intervention on rat acetaldehyde-induced HSC.The cellular mor-phological change was observed by the microscope.The cell proliferation was detected by MTT,the cell cycle was analyzed by flow cytometry(FCM),and the expression of c-myc protein was examined by the SABC method.Results (1)after acetaldehyde stimula-tion,HSC was increased in size and proliferated rapidly,but with the added SB203580 concentration increase,the cellular prolifera-tion was slowed down,the cells size was diminished and the deformed cells were increased.(2)The proliferation of acetaldehyde-in-duced HSC was inhibited by different doses of SB203580,and the higher concentration has the more significant inhibiting effect.(3) With the SB203580 concentration increase,the cells at the phase G0 and G1 were increased,while the cells at the phase S were de-creased,at the same time the expression positive rate of c-myc protein was decreased.Conclusion Blocking p38MAPK pathway ac-tivity could inhibit the proliferation of acetaldehyde-induced HSC,which may be related to the down-regulation of C-myc protein ex-pression and blocking the DNA synthesis in cells entering from G0/G1 phase to S phase.关键词
肝硬化/肝星状细胞/p38MAPK/c-myc基因Key words
liver cirrhosis/hepatic stellate cell/p38MAPK/c-myc gene分类
医药卫生引用本文复制引用
郑人源,张琴,卓强,蒋明德,梅浙川..阻断p38MAPK信号通路对大鼠肝星状细胞活性及c-myc蛋白表达的影响[J].重庆医学,2014,(25):3307-3310,4.基金项目
四川省卫生厅科研项目（130311）；四川省教育厅科研项目（14zb0243）；成都医学院科研项目（CYZ12-016）。 （）
