重庆医学Issue(25)：3319-3321,3.DOI:10.3969/j.issn.1671-8348.2014.25.019
血小板抗原谱细胞的建立在血小板同种抗体检测及鉴定中的应用
Establishment of platelet antigen panel cells and application in platelet alloantibody detection
摘要
Abstract
Objective To establish the platelet antigen panel cells and to apply them in the detection and identification of platelet alloantibody.Methods Human platelet antigen(HPA)1-16 genotyping from 1 500 un-related blood donors in Nanning area were performed by the polymerase chain reaction-sequence specific primers(PCR-SSP)technique,platelet antigen cells with O blood type were chosen to establish the panel cells of platelet antigen.The phenotype of the panel cells were verified by the reference sera from the 14th platelet immunology workshop of the International Society of Blood Transfusion(ISBT).And then these panel cells were used in clinic to detect the platelet alloantibody and the samples from the 14th and 15th platelet immunology workshop of ISBT.Re-sults Six platelet cells with consistent phenotype and genotypes and covering the HPA 1-5 and 1 5 systems were selected to estab-lish the platelet panel cells and successfully applied them in the clinical and scientific sample detection and identification.Conclusion Platelet antigen panel cells are established successfully,which provides the experimental basis for the diagnosis and research of platelet allogenic abnormal immunity diseases.关键词
血小板/谱细胞/建立/抗体/检测Key words
platelets/panel cell/establish/antibody/detection分类
医药卫生引用本文复制引用
周燕,钟周琳,刘金莲,李丽兰,申卫东,吴国光..血小板抗原谱细胞的建立在血小板同种抗体检测及鉴定中的应用[J].重庆医学,2014,(25):3319-3321,3.基金项目
广西科技厅主席资金项目（08160-05）；南宁市科技局项目（201003048C-2）。 （）
