重庆医学Issue(25)：3322-3324,3328,4.DOI:10.3969/j.issn.1671-8348.2014.25.020
小鼠脂肪干细胞分离培养方法
Isolation and culture methods of mouse adipose-derived stem cells
摘要
Abstract
Objective To establish a simple and effective method for isolation and culture of mouse adipose-derived stem cells (mASCs)in vitro,in order to provide the sufficient sources of seed cells for the research of mesenchymal stem cells.Methods The mouse inguinal fat tissues were isolated in vitro and performed a digestion with 0.1% collagenase type NB4,then adipose-derived stem cells(ASCs)were seeded and adhered to the culture dishes in low glucose DMEM containing 10% fetal calf serum.The cellu-lar morphology,in vitro proliferation capacity,multidifferentiation potential and immunophenotype were assessed.Results The mASCs showed good cell morphology,extremely strong proliferation capacity and potential of adipogenesis,osteogenesis and chon-drogenesis via in vitro three-dimensional induction.The cellular surface antigen phenotype was consistent with that reported by lit-erature,and the expression of CD34 and CD105 was positive,Sca-1 was highly expressed,CD45 and SSEA-1 were not expressed. Conclusion Using the experimental methods in this research can culture the high purity of mASCs with the excellent stem cell properties and extremely strong proliferative ability.关键词
小鼠/脂肪干细胞/分化潜能/表面标记Key words
mouse/adipose-derived stem cells/differentiation potential/immunophenotype分类
生物科学引用本文复制引用
蒋婷,杨泽龙,白倩,张兰芳,雷英,周广东,刘伟..小鼠脂肪干细胞分离培养方法[J].重庆医学,2014,(25):3322-3324,3328,4.基金项目
国家自然科学基金资助项目（81301568）。 （）
