重庆医学Issue(28)：3700-3703,4.DOI:10.3969/j.issn.1671-8348.2014.28.002
结肠癌HCT-116细胞增殖及细胞周期的影响
The Effects of SLP-2 on proliferation and cell cycle of HCT-1 1 6 cell line
张剑 1邢鑫 1吴敏 1张自森 1王利娟 1张红巧 1师广勇 1巴楠 1闫琳 1郑晓珂1
作者信息
- 1. 郑州大学第五附属医院肿瘤科 450052
- 折叠
摘要
Abstract
Objective To investigate SLP-2 expression in colorectal cancer and its influence on proliferation and cell cycle of HCT-116 cells.Methods Immunohistochemical method was used to detect the expression of SLP-2 in 50 normal colorectal tissues and colorectal cancer tissue specimens.Then,analysis the relationship between the expression of SLP-2 and the clinical pathological characteristics of the 50 colorectal cancer cases was made.SLP-2 siRNA was transfected into colorectal cancer cell line HCT-116. RT-PCR and Western blot were used to detect the SLP-2 gene expression in HCT-1 1 6 after the transfection.The cell proliferation was detected by MTT,the cell cycles was analyzed by flow cytometry.Results SLP-2 expression in colonic tissue and colorectal cancer tissue were 20% and 70% respectively,which showed statistical significance(P<0.05).The expression of SLP-2 in colorec-tal cancer tissues was associated with TNM stage and lymph nodes metastasis(both P<0.05).After SLP-2 siRNA was interfered, the HCT-116 cells showed slower growth,the number of cells in G1 phase was increased and S phase was declined(both P<0.05), And the expression of SLP-2 in HCT-116 cell was decreased(P<0.05).Conclusion SLP-2 protein shows up-regulation in colorec-tal cancer,and it may have some relationship with carcinogenesis and development of colorectal cancer.关键词
结肠肿瘤/SLP-2/小干扰 RNA/细胞增殖/细胞周期Key words
colonic neoplasms/SLP-2/siRNA/cell proliferation/cell cycles分类
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张剑,邢鑫,吴敏,张自森,王利娟,张红巧,师广勇,巴楠,闫琳,郑晓珂..结肠癌HCT-116细胞增殖及细胞周期的影响[J].重庆医学,2014,(28):3700-3703,4.
