重庆医学Issue(28)：3749-3752,4.DOI:10.3969/j.issn.1671-8348.2014.28.018
二甲双胍抑制Fo xp 3的表达诱导乳腺癌细胞凋亡
Metformin induced apoptosis in breast cancer cell by decreasing the expression of Foxp3
摘要
Abstract
Objective To research the effects of metformin on proliferation and apoptosis in human breast cancer MCF-7 cells and investigate the role of Foxp3 in the effect.Methods The inhibit rate of MCF-7 cells was measured by MTT assay after 48 h metformin treatment(5 mmol/L、10 mmol/L、20 mmol/L).Metformin-induced cell apoptosis was measured by Hoechst 33258 stai-ning and flow cytometry.The expression levels of Foxp3 and caspase-3 mRNA were detected by real-time PCR.Expression of Foxp3 protein was detected by Western blot.Results Metformin markedly inhibited proliferation of MCF-7(P<0.05)compared with control group.And the early apoptotic rates increased to 3.76%,8.96%,and 18.67% with 5 mmol/L、10 mmol/L、20 mmol/L metformin treatment,respectively.Metformin increased caspase-3 mRNA expression levels,and decreased the expression levels of Foxp3 mRNA and protein expression levels(P<0.05,vs.control).Conclusion Metformin could inhibit the proliferation and in-duce apoptosis of MCF-7,and its mechanism maybe related to down-regulated expression of Foxp3.关键词
二甲双胍/乳腺肿瘤/细胞凋亡/Foxp3Key words
metformin/breast neoplasms/apoptosis/Foxp3分类
医药卫生引用本文复制引用
黄文君,陈健,田晶..二甲双胍抑制Fo xp 3的表达诱导乳腺癌细胞凋亡[J].重庆医学,2014,(28):3749-3752,4.基金项目
国家自然科学基金资助项目（81260343）；广西教育厅科研基金资助项目（2013YB159）。 （）
