重庆医学Issue(29)：3901-3903,3906,4.DOI:10.3969/j.issn.1671-8348.2014.29.018
新生大鼠皮层神经元体外无血清原代培养
Establishing a serum-free primary culture method for cortical neurons of new-born rats
摘要
Abstract
Objective To establish a serum-free primary culture method for cortical neurons of new-born rats .Methods The cortical tissue was digested and the cells were planted in the medium containing 10% fetal bovine serum ,and then maintained feed-ing with neurobasal medium containing B27 after 4 to 8 h .The morphology was observed under phase-contrast microscope .RT-PCR ,Western blot and immunocytochemistry were applied to identify the expression of NSE gene and protein in neurons .Results A large number of neurons began to adhere to the cover glasses after 2 to 8 h .They showed different shapes-shuttle ,triangle pyram-idal ,or no regular after clinging to the plate .Their processes connected to nets and were different in length and thickness .They well developed at the 7th to 10th day .The isolated and cultured cells were confirmed as neurons by RT-PCR ,Western blot and immuno-cytochemistry .Conclusion This technique is an easy and practical tool for primary culture of new-born rats cortical neurons with high purity ,and can be used as an in-vitro model of research .关键词
神经元/细胞 ,培养/培养基/细胞模型Key words
neuron/ceus ,cultured/delture media/cell model分类
医药卫生引用本文复制引用
杨传豪,王业忠,赵冬,刘祺,姬云翔,李令建,许晖,戴晶,何学君,蔡云鹏..新生大鼠皮层神经元体外无血清原代培养[J].重庆医学,2014,(29):3901-3903,3906,4.基金项目
新疆生产兵团博士基金资助项目（2011BB016）。 （）
