重庆医学Issue(29)：3932-3934,3.DOI:10.3969/j.issn.1671-8348.2014.29.029
五味子甲素SchA缓解MPP＋诱导的SH-SY5Y细胞损伤的机制研究
Investigation of mechanism of SchA to decreasing MPP+-induced SH-SY5Y cell damage
摘要
Abstract
Objective To explore the possible mechanism of SchA ,which decreases MPP+induce SH-SY5Y cell damage .Meth-ods Cultured cells were divided into 5 groups ,one as control group ,cultured by free-blood serum media;the other 4 groups were treated with different concentrations of SchA(1 ,3 ,5 μmol/L) and MPP+ (1 mmol/L) for 48 h named model group ,1 ,3 ,5 μmol/L SchA group respetivly .The content of nitric oxide(NO) were measured by NO kit ;The expression levels of total Akt and p-Akt proteins were detected by Western blot .Results Compared with the control group ,the content of NO in group significantly in-creased after MPP+stimulating(P<0 .05);compared to the control group ,the content of NO in 5μmol/L SchA group significantly decreased(P<0 .05) .The expression levels of total Akt in all groups had no significant difference(P>0 .05) .The expression levels of p-Akt in model group significantly lowered ,while SchA(1、3、5 μmol/L) significantly increased the expression levels of p-Akt in comparision with cells in model group .Conclusion Decreasing MPP+ induced SH-SY5Y cell damage of SchA may be related to the content of NO and p-Akt expression .关键词
五味子素/1-甲基-4-苯基吡啶离子/S H-SY5Y细胞/蛋白激酶类Key words
schizandrin/MMP+/SH-SY5Y cell/protein kinases分类
医药卫生引用本文复制引用
纪朋艳,李妍,张巍,彭顺利,何哲..五味子甲素SchA缓解MPP＋诱导的SH-SY5Y细胞损伤的机制研究[J].重庆医学,2014,(29):3932-3934,3.基金项目
吉林省科技厅科研项目（201205079）；吉林市科技局科研项目（201162511）。 （）
