重庆医学Issue(30)：4065-4067,4070,4.DOI:10.3969/j.issn.1671-8348.2014.30.028
贵州地区丙型肝炎病毒基因分型的研究
Study on hepatitis C virus genotyping in guizhou area
摘要
Abstract
Objective To study the distribution of genotypes of hepatitis C virus in Guizhou and its relationship between infec-tious route of genotype and age ,gender was analyzed .Methods Serum specimens in this study were obtained from 198 patients , whose anti-HCV and HCV RNA were positive .A reverse transcriptase PCR(RT nested-PCR)assay using conserved primers de-duced from the core-envelope 1(C-E1)region of the hepatitis C virus(HCV)genome was employed to amplify a 474-nucleotide-long fragment .Phylogenetic analysis of the C-E1 sequences was conducted by direct sequencing of the RT-PCR products and alignment with published HCV subtypes in GenBank .Subtypes of the samples were determined by nucleotide sequencing followed by composi-tion of a phylogenetic tree .Results Among the 198 patients surveyed ,genotype 1a was detected in 4 cases(2 .0% ) ,genotype 1b in 71 cases(35 .9% ) ,genotype 2a in 9 cases(4 .6% ) ,genotype 3a in 29 cases(14 .7% ) ,genotype 3b in 47 cases(23 .7% ) ,genotype 6 a in 37 cases(18 .7% )and genotype 6d in 1 cases(0 .5% ) .Genotype distribution on gender had no statistical significance(P>0 .05) , and its distribution on people with different ages had statistical significance(P<0 .05) ,and its distribution on patients with different infectious routes was significantly different(P<0 .05) .Conclusion The major genotypes of HCV are 1b ,3b ,6a and 3a in Guizhou , and genotype 1a is predominant .Genotypes 1a ,2a and 6d exist too .Genotypes of patients infected with HCV are related to their in-fectious routes ,and the HCV genotypes are in a great variety .关键词
贵州肝炎病毒 ,丙型/基因型/研究Key words
guizhou hepatitis C virus/genotype/research分类
医药卫生引用本文复制引用
杨兴林,梁跃东,李勇富,张流,熊金凤,王义光,黄海..贵州地区丙型肝炎病毒基因分型的研究[J].重庆医学,2014,(30):4065-4067,4070,4.基金项目
贵州省科教英才基金［黔省专合字（2012）179号］；贵阳市科技局计划项目［筑科合（2012103）9号］。 （）
