重庆医学Issue(31)：4175-4178,4.DOI:10.3969/j.issn.1671-8348.2014.31.019
EDA-A1基因突变体对人脐静脉内皮细胞 ECV304增殖及细胞周期的影响
Effect of EDA-A1 gene mutant on proliferation and cell cycle distribution of cultured human umbilical vein endothelial cell ECV304
雷科 1王伦昌 1李龙江 2车团结 3何祥一4
作者信息
- 1. 四川省广元市中心医院口腔科 628000
- 2. 四川大学口腔疾病重点研究实验室，成都 610000
- 3. 兰州大学生命科学院细胞生物学研究所，兰州 730000
- 4. 兰州大学口腔医学院口腔修复科，兰州 730000
- 折叠
摘要
Abstract
Objective To investigate the effect of ectodysplasin A (EDA‐A1) gene of hypohidrotic ectodermal dysplasia on pro‐liferation and cell cycle of human umbilical vein endothelial cell (ECV304). Methods Recombinant eukaryotic expression vectors pcDNA3. 1(‐)‐EDA‐A1‐M /W (mutant, M and wild, W) containing the coding sequence were transected into ECV304 cells. EDA‐A1 gene was amplified by reverse transcription polymerase chain reaction (RT‐PCR), and the protein was detected by Western blot. Cell viability and cycle distribution were invested by MTT and Flow cytometry (FCM ). Results The EDA‐A1 gene and pro‐tein were detected respectively by RT‐PCR and Western blot in ECV cells transfected with pcDNA3. 1(‐)‐EDA‐A1‐M /W, but not in ECV cells transfected with plasmid pcDNA3. 1(‐) and cells without transection. And also, compared with control groups, EDA‐A1 gene mutant significantly decreased proliferation of ECV cells and its inhibition rate was 45. 70% ( P < 0. 01), whereas wild EDA‐A1 gene did not cause such growth inhibition (P> 0. 05). A significant increase of the G0 /G1 and S fraction was seen in the ECV cells of mutant group, compared with wild group with an accumulation in S phase and a concomitant decrease in G2 /M phase population (P< 0. 05). Conclusion Mutant and wild EDA‐A1 gene may have distinct biological functions on proliferation and cell cycle distribution of cultured human umbilical vein endothelial cell.关键词
细胞周期/细胞增殖/EDA-A1 基因/突变体/人脐静脉内皮细胞Key words
cell cycle/cell proliferation/EDA-A1 gene/mutant/human umbilical vein endothelial cell分类
医药卫生引用本文复制引用
雷科,王伦昌,李龙江,车团结,何祥一..EDA-A1基因突变体对人脐静脉内皮细胞 ECV304增殖及细胞周期的影响[J].重庆医学,2014,(31):4175-4178,4.
