重庆医学Issue(33)：4422-4424,3.DOI:10.3969/j.issn.1671-8348.2014.33.002
不同剂量吉非替尼对博莱霉素所致大鼠肺纤维化影响的比较
Comparison of the influence of different doses of gefitinib on the rat pulmonary fibrosis induced by bleomycin
摘要
Abstract
Objective To compare the effect of different doses of gefitinib on the extent of fibrosis in rat models of pulmonary fi‐brosis induced by bleomycin (BLM) .Methods Seventy five female rats were randomly assigned to five groups and treated as fol‐lows :the rats in control group were given water orally with administration of saline intratracheally ;the rats in BLM group were giv‐en water orally with a single intratracheal injection of BLM (5 mg/kg body weight in 0 .2 mL phosphate buffered saline);the rats in BLM+Ge20 group was given gefitinib at dose of 20 mg・ kg -1 ・ d-1 orally with administration of bleomycin intratracheally ;the rats in BLM+Ge90 group were given gefitinib at dose of 90 mg・kg -1 ・d-1 orally with administration of BLM intratracheally ;the rats in BLM+Ge200 group were given gefitinib at dose of 200 mg・kg -1 ・d-1 orally with administration of BLM intratracheally . Twenty one days later ,all rats were sacrificed .Left pulmonary lobes from all animals were removed and stained with hematoxylin , eosin and Masson′s trichrome respectively for pathological examination ,and the right pulmonary lobe tissues were used for the hydroxyproline contents measurement (by ELISA ) .Results Gefitinib augumeted rat pulmonary fibrosis induced by bleomycinin with significantly increasing Ashcroft score ,lung collagen accumulation and the level of hydroxyproline (HYP) in BLM + Ge200 group (P<0 .05) .The hydroxyproline content of pulmonary tissue was increased in BLM+Ge90 group conmpared to those in BLM group (P<0 .05) ,while Ashcroft score was not increased .However ,there was no significant difference in the indicators above be‐tween the BLM+Ge20 group and the BLM group (P>0 .05) .Conclusion Gefitinib augumets rat pulmonary fibrosis induced by bleomycin .The bigger the gefitinib dosage is ,the more severe pulmonary fibrosis would be .关键词
肺纤维化/大鼠/博莱霉素/吉非替尼Key words
pulmonary fibrosis/rats/bleomycin/gefitinib分类
医药卫生引用本文复制引用
黄文亭,林采余,韩睿,王玉波,陈恒屹,卢从华,李力,何勇..不同剂量吉非替尼对博莱霉素所致大鼠肺纤维化影响的比较[J].重庆医学,2014,(33):4422-4424,3.基金项目
国家自然科学基金资助项目（81071912，81172113）；全军医学科技青年培育项目（13QNP117）。 （）
