重庆医学Issue(33)：4447-4449,4453,4.DOI:10.3969/j.issn.1671-8348.2014.33.010
AAV-HGFK1抑制EGFR磷酸化拮抗大肠癌细胞生长
AAV-HGFK1 inhibits proliferation of colorectal cancer cells by down-regulating the phosphorylation of EGFR
邓飞鸿 1聂飚 1左俊华 1柳雪花 1陈金敏1
作者信息
- 1. 南方医科大学南方医院消化科，广州510515
- 折叠
摘要
Abstract
Objective To study the effect of adeno associated virus hepatocyte growth factor K1(AAV‐HGFK1)on the prolifer‐ation of 4 different colorectal cell lines with or without KRAS or BRAF mutation .Methods The levels of epidermal growth factor receptor (EGFR) mRNA were determined in SW48 without KRAS or BRAF mutation ,Lovo with KRAS mutation ,SW620 with KRAS mutation ,HT29 with BRAF mutation by quantitative real time PCR ,respectively .After the infection of AAV‐HGFK1 ,the expressions of EGFR ,p‐EGFR and β‐actin were detected by Western blot and the proliferation of the cells were assayed using MTT .Results Lovo ,SW48 and HT29 expressed EGFR protein while SW620 did not .EGF promoted the proliferation of Lovo , SW48 and HT29 cells .AAV‐HGFK1 down‐regulated the phosphorylation of EGFR and significantly inhibited their proliferation . But EGF had no effect on proliferation of SW620 stimulated by EGF .Conclusion AAV‐HGFK1 exhibited its antitumor effects through EGF/EGFR signaling irrespective of the KRAS or BRAF mutation and may also act through other signaling pathways .关键词
受体,表皮生长因子/大肠肿瘤/肝细胞生长因子K1/腺相关病毒Key words
receptor ,epidermal growth factor receptor/colorectol neoplasms/hepatocyte growth factor K1/adeno associated virus分类
医药卫生引用本文复制引用
邓飞鸿,聂飚,左俊华,柳雪花,陈金敏..AAV-HGFK1抑制EGFR磷酸化拮抗大肠癌细胞生长[J].重庆医学,2014,(33):4447-4449,4453,4.
