重庆医学Issue(33)：4494-4497,4.DOI:10.3969/j.issn.1671-8348.2014.33.026
采用Changliver细胞制备肝细胞脂肪变性模型的方法探索
To explore the method of establishing hepatocellular steatosis models by Changliver cell
摘要
Abstract
Objective To explore the experimental condition for hepatocellular steatosis models of Changliver cell induced by o‐leic acid (lleic acid ,OA) .Methods Changliver cells were induced by different concentration of oleic acid for different periods .MTT was used to detect hepatic cell activity ,oil red 0 staining was used to observe intracellular lipid droplets acumulation ,glycerin 3 phosphate oxidase method was applied to detect the contents of triglyceride (TG) in the Changliver cell .Results Hepatocellular steatosis models of Changliver cell can be established successfully by 0 .2 mmol/L OA inducing for 24 hours .TG content in model cells was (379 .98 ± 23 .19)mg/g ,however ,it was (185 .03 ± 12 .68)mg/g in control cells ,the difference was statistically significant (P< 0 .01) .Conclusion The proper condition for establishing hepatocellular steatosis models is 0 .2 mmol/L OA inducing Changliver cells for 24 h .This model is the reliable choice for nonalcoholic fatty liver disease research .关键词
脂肪肝/模型/油酸/Changliver细胞Key words
fatty liver/model/oleic acid/Changliver cells分类
医药卫生引用本文复制引用
阿卜力克木·奥布力,程磊,黄凤玲,闫冬,迪丽阿热姆·尼加提,帕尔哈提·克热木,魏媛媛..采用Changliver细胞制备肝细胞脂肪变性模型的方法探索[J].重庆医学,2014,(33):4494-4497,4.基金项目
国家自然科学基金资助项目（81160546）；大学生创新性实验计划（CX2012030）。 （）
