重庆医学Issue(34)：4574-4578,5.DOI:10.3969/j.issn.1671-8348.2014.34.005
CD147在膀胱尿路上皮癌组织中的表达及临床意义
Expression and clinical significance of extracellular matrix metalloproteinase inducer molecule CD1 4 7 in bladder urothelial carcinoma tissue
刘敏 1毛向明 2薛义军 1邹晓峰 3袁源湖 3肖日海 3张国玺 3伍耿青 3王晓宁3
作者信息
- 1. 南方医科大学附属南方医院泌尿外科，广州 510515
- 2. 赣南医学院第一附属医院泌尿外科，江西赣州 331000
- 3. 赣南医学院第一附属医院泌尿外科，江西赣州 331000
- 折叠
摘要
Abstract
Objective To investigate the expression of extracellular matrix metalloproteinase inducer CD147 in patients with bladder urothelial carcinoma and its relationship with clinicopathologic features and prognosis.Methods RT-PCR and Western blot were used to evaluate the mRNA and protein expression of CD147 in cancer tissues and corresponding paracanerous tissues from 20 patients with bladder urothelial carcinoma;tissue microarray containing specimens from 108 patients with bladder urothelial carcino-ma and 20 normal controls was constructed and immunohistochemistry was applied to examine the expression of CD147,and the correlations between CD147 and clinicopathological characteristics and prognosis were also analyzed.Results Expression level of CD147 mRNA and protein in cancer tissues from 20 bladder urothelial carcinoma patients was significantly higher than that of cor-responding adjacent tissues(P<0.01).Immunohistochemical detection showed normal bladder urothelium from 20 control cases were either no or weak protein expression of CD147,positive expression rate of CD147 in 108 cases of bladder urothelial carcinoma was 73.1%.the CD147 expression level had positive correlation with lymph nodes metastasis,histological grade,tumor stage and tumor size of patients with bladder urothelial carcinoma(P<0.05)and had no relationship with age,sex,the number of tumor(P>0.05).Kaplan-Meier survival curves analysis showed that the survival time of patients with bladder urothelial carcinoma whose ex-pression of CD147 were high was significant lower than that of patients with bladder urothelial carcinoma whose expression of CD147 were low(P<0.01).Univariate analysis showed that histological grade,tumor stage,lymph node metastasis,tumor size and CD147 expression were all positively correlated with a poor prognosis(P<0.05).Cox-multivariate analysis revealed that histologi-cal grade,tumor stage,lymph node metastasis,and CD147 expression level were independent predictors of an unfavorable prognosis (P<0.05).Conclusion High expression of CD147 in bladder urothelial carcinoma may be correlate with the its progress and its expression state is an independent prognosis factor.关键词
膀胱肿瘤/CD147/组织芯片Key words
urinary bladder neoplasms/CD147/tissue microarray分类
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刘敏,毛向明,薛义军,邹晓峰,袁源湖,肖日海,张国玺,伍耿青,王晓宁..CD147在膀胱尿路上皮癌组织中的表达及临床意义[J].重庆医学,2014,(34):4574-4578,5.
