重庆医学Issue(35)：4705-4707,3.DOI:10.3969/j.issn.1671-8348.2014.35.001
霍奇金淋巴瘤细胞SHP1表达与CD99表达的关系
The relationship between the expression of SHP1 and CD99 in Hodgkin lymphoma cell
摘要
Abstract
Objective To explore the relationship between SHP1 and CD99 expression in Hodgkin lymphoma (HL) .Methods RT‐PCR and Western blot to detect the expression of CD99 and SHP1 mRNA and protein expression in IM9、KM3、L428、L428‐CD99 cells ;fluorescence confocal to observe the expression and co‐localization of CD99 and SHP1 ;transiently interference CD99 in IM9 cells and then detect the expressing of SHP1 mRNA and protein levels .Results SHP1 mRNA was low expressed in L428、KM3 and high expressed in IM9;gene and protein expression were consistent trend;in L428‐CD99 cell SHP1 mRNA expression and protein levels were higher than L428 cell;CD99 expressed in membrane and SHP1 expressed in cell plasma;transient interference CD99 ,SHP1 decreased in mRNA expression and protein levels .Conclusion In HL cells ,SHP1 expression related to the missing expression of CD99 ,and its mechanism remains to be studied .关键词
SHP1/CD99/霍奇金淋巴瘤Key words
SHP1/CD99/Hodgkin lymphoma分类
医药卫生引用本文复制引用
雷翔慧,汤瑶,吴自勍,赵彤..霍奇金淋巴瘤细胞SHP1表达与CD99表达的关系[J].重庆医学,2014,(35):4705-4707,3.基金项目
国家自然科学基金资助项目（81071659）。 （）
