重庆医学Issue(35)：4711-4714,4.DOI:10.3969/j.issn.1671-8348.2014.35.003
LPS和LiCl预处理对LPS肝损伤保护作用的研究
Protective effect of LPS and LiCl pretreatment on endotoxin liver injury
摘要
Abstract
Objective To compare protective effect on lipopolysaccharide (LPS)‐induced liver injury by LPS tolerance with that of glycogen synthase kinase‐3 (GSK‐3) inhibitor LiCl .Methods Thirty‐six male healthy Sprague‐Dawley rats (200 ± 10)g were randomly divided into control group ,LPS tolerance groupand LiCl treatment group ,12 rats in each group;the LPS tolerance group was conducted intraperitoneal injection at 0 .015 ,0 .030 and 0 .060 mg/kg (weight) on the first ,second and third day ;LiCl treat‐ment group was also received intraperitoneal injection 0 .25 mL at 2 .5 mol/L LiCl at the same time point ;the control group was in‐traperitoneally injected PBS 0 .25 mL every day ;after pretreatment ,each group was divided into the LPS(-) and LPS(+ ) sub‐groups on the basis that whether lethal dose LPS(10 mg/kg) was accepted .LPS(+ ) subgroup received 10 mg/kg (weight) LPS in‐traperitoneal injection ,and LPS(-) subgroup took the same volume of PBS intraperitoneal injection .After 12 h continuous obser‐vation ,the rats were anaesthetized ,killed when alive and drawn the materials .Observed general pathological changes of liver;patho‐logical changes were observed under light microscope and electron microscope;then the content of glutamic‐pyruvic transaminase (ALT) ,lactate dehydrogenase (LDH) and total bilirubin(TB) of plasma was detected quantitatively ;the content of IL‐10/TNF‐αin serum was measure .Results After LPS attack ,liver histopathology damage was caused to change ,liver function indicators ALT , LDH and TB of control group increased significantly ,TNF‐α and IL‐10 grew plasma;the damaged changes of liver tissue induced could be improved by LPS and LiCl pretreatmen ,as well as the liver function ,and inhibit the increase of TNF‐αand raise plasma IL‐10 .Conclusion Intraperitoneal injection of LPS at a large dose can induce liver damage ,and LPS tolerance and GSK‐3 inhibitor LiCl have similar liver protection effect .关键词
内毒素类/大鼠 ,Sprague-Dawley/糖原合成酶激酶-3/肝损伤Key words
endotoxins/rat ,Sprague-Dawley/glycogen synthase kinase 3/liver injury分类
医药卫生引用本文复制引用
胡中生,张艳,徐发良,龚建平,朱小林..LPS和LiCl预处理对LPS肝损伤保护作用的研究[J].重庆医学,2014,(35):4711-4714,4.基金项目
重庆市科委资助项目（cstcjjA ）。 （）
