重庆医学Issue(35)：4723-4726,4.DOI:10.3969/j.issn.1671-8348.2014.35.006
亚硒酸钠对人增生性瘢痕成纤维细胞体外增殖的影响
Effect of sodium-selenite on human hypertrophic scar fibroblasts proliferation in vitro
杨陆涛 1刘美玲 1张友来 1辛国华 1李国辉 1曾元临1
作者信息
- 1. 南昌大学第一附属医院烧伤中心，南昌 330006
- 折叠
摘要
Abstract
Objective To observe the effects of sodium‐selenite on human hypertrophic scar fibroblasts proliferation in vitro . Methods Human hypertrophic scar fibroblast culture was conducted in vitro ,the status of fibroblast proliferation of the 4th gener‐ation cells was tested by CCK‐8 ,which was divided into experimental group and control group ,the experimental group was divided into six groups (A ,B ,C ,D ,E ,F) ,and were added an equal volume‐containing 2 .5 ,5 .0 ,10 .0 ,20 .0 ,40 .0 ,80 .0 μmol/L concentra‐tions of sodium selenite in 10% FBS culture medium ;the control group added an equal volume of 10% FBS culture medium ,testing cell proliferation by CCK‐8 at 24 ,48 ,72 ,96 h respectively ;testing different concentrations of sodium‐selenite cell survival situation after 24 h by Live/dead reagent ;immunohistochemical was used to test intracellularⅠ ,Ⅲ type collagen expression after 24 h .Re‐sults (1)With the increased of concentration ,the inhibition rate of fibroblasts gradually increased as the concentration of sodium‐selenite ranged in 2 .5-80 .0 μmol/L(P<0 .05);(2) the inhibition rate of sodium‐selenite on fibroblasts gradually increased at the same concentration with time(P<0 .05);(3)Live/dead reagent test results showed that apoptosis cell number increased with the concentration increasing ;(4 ) With concentrations of sodium‐selenite increasing ,typeⅠ ,Ⅲ collagen expression of fibroblast de‐creased gradually .Conclusion Sodium‐selenite can inhibit human hypertrophic scar fibroblast proliferate in vitro and reduce Ⅰ ,Ⅲcollagen expression of fibroblast type.关键词
亚硒酸钠/瘢痕/成纤维细胞/增殖Key words
sodium nitrite/cicatrix/fibroblast/proliferation分类
医药卫生引用本文复制引用
杨陆涛,刘美玲,张友来,辛国华,李国辉,曾元临..亚硒酸钠对人增生性瘢痕成纤维细胞体外增殖的影响[J].重庆医学,2014,(35):4723-4726,4.
