重庆医学Issue(1)：1-3,6,4.DOI:10.3969/j.issn.1671-8348.2015.01.001
4-苯乙烯磺酸马来酸酐共聚物抑制HIV-1的初步研究
The preliminary research of PSM on the inhibition of HIV-1
摘要
Abstract
Objective To investigate the inhibiton effect of 4‐styrenesulfonic acid‐co‐maleic acid(PSM ) in HIV‐1 .Methods The inhibition effect of different doses of PSM on HIV‐1 in susceptible cells GHOST (3) X4/Hi5 was observed by Luciferase ,and so did the inhibitory effect of PSM on JR‐FL、HXB2、CNE6 ,CNE30 ,CNE50 ,CNE55 .The cellular toxicity of PSM on the VK2/E6E7 was also evaluated by CCK8 kit .The transcript level of tight junction proteins (ZO‐1 ,E‐cadherin and Occludin) of HEC‐1‐A were analyzed by qRT‐PCR .And then observed the effect of PSM on expression of genitourinary epithelial cells HEC‐1‐A ,so we could e‐valuated the effect of integrity of local mucosal indirectly .Results The results showed that PSM exhibited potent antiviral activity against a broad spectrum of HIV‐1 major isolates with different genotypes and biotypes (EC50 value of JR‐FL ,HXB2 ,CNE6 , CNE30 ,CNE50 ,CNE55 were 5 .78 ,0 .77 ,1 .85 ,3 .15 ,1 .70 ,2 .27 μg/mL respectively) .Meanwhile ,it had less cytotoxicity on VK2/E6E7 .qRT‐PCR showed that no obvious restrain effect on expression of ZO‐1 was observed and PSM increased the level of tran‐scription of E‐cadherin and Occludin .Conclusion PSM may be a potential agent for the prevention of HIV‐1 infection .关键词
4-苯乙烯磺酸马来酸酐共聚物/抗病毒/HIV-1Key words
4-styrenesulfonic acid-co-maleic acid/antiviral/HIV-1分类
医药卫生引用本文复制引用
张龙锋,邱敏,蒋丽军,刘强,焦志军,吴稚伟..4-苯乙烯磺酸马来酸酐共聚物抑制HIV-1的初步研究[J].重庆医学,2015,(1):1-3,6,4.基金项目
国家卫计委“十二五”传染病重大专项项目（2012ZX10001007-009）；江苏大学医学临床科技发展项目基金资助项目（JLY20120169）。 （）
