重庆医学Issue(1)：10-13,4.DOI:10.3969/j.issn.1671-8348.2015.01.004
人21．5kDaMBP基因沉默对神经胶质瘤细胞增殖与凋亡的作用研究
Effects of the human 21 .5 kDa MBP gene silencing on the proliferation and apoptosis of glioma cells
摘要
Abstract
Objective To investigate the effects of silencing of the human cerebral 21 .5 kDa myelin basic protein (MBP) gene on the proliferation and apoptosis of the glioma U251 cells .Methods The 21 .5 kDa MBP sequence‐specific short hair‐pin RNA (shR‐NA) recombinant plasmids pGenesil‐1‐MBP‐3 were transfected into the human glioma cell line(U251) ,the cells of U251 was used as MBP silencing group ,the cells transfected with negative control plasmids used as negative control group ,and the cells transfected with liposomes used as blank control group .Real‐time PCR and Westernblot were used to detect the expression levels of the 21 .5 kDa MBP mRNA and protein in each group ,and the cell proliferation curve was measured by CCK‐8 assay ,the apoptosis rate was a‐nalysised by Flow cytometry .Results Both the mRNA and the protein expression levels of the 21 .5 kDa MBP of MBP silencing group were significantly lower than those in the control groups (P<0 .05);the cellular proliferation activity of the MBP silencing group decreased significantly (P<0 .05)while the cellular apoptotic rate increased significantly (P<0 .05) .Conclusion Silencing of the human cerebral 21 .5 kDa MBP gene may playa dual role in the inhibition of proliferation and the promotion of apoptosis of the glioma U251 cells .关键词
RNA干扰/基因沉默/转染/21.5kDaMBP/神经胶质瘤/增殖/凋亡Key words
RNA interference/genes silencing/transfection/21 .5 kDa MBP/glioma/proliferation/apoptosis分类
医药卫生引用本文复制引用
田瑞敏,王含彦,易芳,王晓明,陈建业..人21．5kDaMBP基因沉默对神经胶质瘤细胞增殖与凋亡的作用研究[J].重庆医学,2015,(1):10-13,4.基金项目
四川省教育厅重点课题基金资助项目（2006J13031）；川北医学院2013年青年项目资助（CBY13-A-QN33）。 （）
