重庆医学Issue(2)：186-188,191,4.DOI:10.3969/j.issn.1671-8348.2015.02.014
甲基化敏感性高分辨率熔解曲线分析筛查大肠癌的性能评价
Colorectal cancer screening by utilizing methylation-sensitive high-resolution melting curve analysis
摘要
Abstract
Objective To assess the performance of the methylation‐sensitive high‐resolution melting curve analysis (MS‐HRM analysis) on the detection of the methylation in stool DNA for colorectal cancer screening .Methods Eighty‐two qualified stool samples were collected from 27 patients with colorectal cancer patients (CRC group) ,25 patients with advanced adenomas (AA group) ,and 30 healthy people (control group) .The methylation status of vimentin gene in all of the stool samples was detected by the MS‐HRM analysis on the LightCycler 480 platform .The fecal occult blood test (FOBT) was also used for the same samples . Results The positive rates of the MS‐HRM assay in the CRC group ,AA group ,and control group were 81 .5% (22/27) ,80 .0%(20/25) ,and 6 .7% (2/30) respectively .The positive rates of FOBT in the three groups were 37 .0% (10/27) ,12 .0% (3/25) and 3 .3% (1/30) respectively .The diagnostic sensitivity of the MS‐HRM assay for colorectal cancer and advanced adenomas (80 .8% , 42/52) was significantly higher than that of FOBT (25 .0% ,13/52)(P<0 .05) .No significant difference was found in the diagnos‐tic specificity between the MS‐HRM assay (93 .3% ,28/30) and the FOBT (96 .7% ,29/30) (P>0 .05) .Conclusion MS‐HRM performs better than FOBT and has great application potential in the detection of stool DNA methylation for colorectal cancer screening .关键词
甲基化/肿瘤/波形蛋白/DNA/进展期腺瘤/甲基化敏感性高分辨率熔解曲线分析Key words
methylation/neoplasms/vimentin/DNA/advanced adenomas/methylation-sensitive high-resolution melting分类
医药卫生引用本文复制引用
肖著军,萧著玲,陈金敏,邓飞鸿,许岸高..甲基化敏感性高分辨率熔解曲线分析筛查大肠癌的性能评价[J].重庆医学,2015,(2):186-188,191,4.基金项目
惠州市科技计划项目（2013y122）。 （）
