重庆医学Issue(3)：293-294,298,3.DOI:10.3969/j.issn.1671-8348.2015.03.002
幽门螺杆菌感染小鼠胃黏膜组织细胞因子变化的实验研究
Experimental study of cytokines in gastric mucosa of mice with Helicobacter pylori infection
摘要
Abstract
Objective To study the change of cytokines in gastric mucosa of mice with Helicobacter pylori infection and explore the immune mechanism .Methods Twenty‐four 8-10 weeks‐old BALB/c mice were randomly divided into observation group and control group .After first 12 hours of fasting ,observation group was given 0 .5 mL/d Helicobacter pylori bacterial liquid with con‐centration of 2 × 109 CFU/mL for 7 consecutive days .The control group was fed with 0 .5 mL/d saline for 7 consecutive days .Then the mice were infected with Helicobacter pylori gast model was established .Using Real‐time PCR to detect the expressions of IFN‐γ ,TNF‐α,IL‐8 and IL‐10 in murine plasma within 2 ,4 weeks after infection .And after 4 weeks of infection ,mice were sacrificed , and their stomach tissue sterile were separated .ELISA was used to detect the levels of IFN‐γ,TNF‐α,IL‐8 ,and IL‐10 mRNA in gastric mucosa .Results There was significantly difference between groups 2 weeks after infection(P<0 .05) .With lastingness of infection ,the expression and the mRNA of IFN‐γ,TNF‐α,IL‐8 were significantly increased ,and the rate of increase of observation group was higher(P<0 .05) .Conclusion TH2 cells may have no effect on Helicobacter pylori infection in mice gastric mucosa and promote the expression of T H1‐type cytokines ,triggering immune response of T H1 .关键词
螺杆菌 ,幽门/胃黏膜/细胞因子类/感染Key words
Helicobacter pylori/gastric mucosa/cytokines/infection分类
医药卫生引用本文复制引用
韩岩智,刘浩,阮开学..幽门螺杆菌感染小鼠胃黏膜组织细胞因子变化的实验研究[J].重庆医学,2015,(3):293-294,298,3.基金项目
国家自然科学基金资助项目（49201065）。 （）
