重庆医学Issue(3)：315-317,3.DOI:10.3969/j.issn.1671-8348.2015.03.009
异甘草素诱导人卵巢癌细胞株SKOV3凋亡及自噬的研究
Pro-apoptosis and pro-autophagy effect of isoliquiritigenin on human ovarian cancer cell lines SKOV3
摘要
Abstract
Objective To investigate the proliferation‐inhibition activity ,pro‐apoptosis and pro‐autophagy of isoliquiritigenin on ovarian cancer cell lines SKOV3 in vitro ,and explore its mechanism .Methods The cytotoxic effect of Isoliquiritigenin(ISL) on the SKOV3 cells was tested by the means of MTT .Cell morphology was observed with invert microscope after AO/EB staining .The apoptosis of the SKOV3 cells was detected with flow cytometer .The expression levels of Bax ,Bcl‐2 ,Beclin1 and LC3 were detected by Western‐blotting .Results Different concentrations of ISL(5 ,10 ,20 μg/mL) showed the inhibitory effect on SKOV3 cells and presented the characteristic morphological changes of apoptosis under inverted microscope .The FCM analysis showed that the ap‐optosis rate of the SKOV3 cells was increased after treatment of ISL accompanied a concentration increment and a prolongation of the culture time(P< 0 .05) .Western‐blotting result showed that the expression levels of Bax ,Beclin1 and LC3 were increased in SKOV3 treated with ISL (5 ,10 ,20 μg/mL) ,while the expression level of Bcl‐2 was significantly decreased (P<0 .05) .Conclusion ISL could inhibit the proliferation of the SKOV3 cells in vitro and induce apoptosis ,and that maybe associate with the mitochon‐drial pathway of apoptosis and autophagy pathway .关键词
卵巢肿瘤/异甘草素/人卵巢癌SKOV3细胞/凋亡/自噬Key words
ovarian cancer/isoliquiritigenin/the SKOV3 cells/apoptosis/autophagy分类
医药卫生引用本文复制引用
张栋栋,付亚杰,陈小芸,鲁永婷,范维,郭钰珍..异甘草素诱导人卵巢癌细胞株SKOV3凋亡及自噬的研究[J].重庆医学,2015,(3):315-317,3.基金项目
兰州大学第二医院院内基金资助（YJzy2013-08）。 （）
