重庆医学Issue(4)：462-464,3.DOI:10.3969/j.issn.1671-8348.2015.04.010
Slit2蛋白对TNF-α诱导的大鼠胸主动脉血管平滑肌细胞增殖迁移的影响
The effects of Slit2 protein on proliferation and migration of rats vascular smooth muscle cells stimulated by TNF-α
刘丽华 1刘涛 2王浩宇 2陈桂秀 2倪伟 2邓学云3
作者信息
- 1. 川北医学院第二临床医学院/四川省南充市中心医院老年病科 637000
- 2. 川北医学院第二临床医学院/四川省南充市中心医院心内科 637000
- 3. 川北医学院第二临床医学院/四川省南充市中心医院神经外科 637000
- 折叠
摘要
Abstract
Objective To observe the effects of Slit2 protein on the proliferation and migration of VSMCs .Methods The VSMCs was cultured in our laboratory .The experiment was divided into two parts ,part one:VSMCs were divided into normal con‐trol group and experimental groups(culture with 50 ,75 ,100 ,125 and 150 ng/mL Slit2 respectively);part two :VSMCs were divided into normal control group ,positive control group(culture with TNF‐α10 ng/mL) and experimental groups(culture with TNF‐α10 ng/mL+Slit2 50 ng/mL ,TNF‐α10 ng/mL+Slit2 75ng/mL ,TNF‐α10 ng/mL+ Slit2 100 ng/mL ,TNF‐α10 ng/mL+ Slit2 125 ng/mL and TNF‐α10 ng/mL+Slit2 150 ng/mL respectively) .To detect proliferation and migration of VSMCs by CCK‐8 and tran‐swell experiment .Results The difference of OD value and numbers of VSMCs has no statistical significance in the presence of Slit 2 (P=0 .516 ,P=0 .52) .The numbers of VSMCs has statistical significance between control and positive control groups (P=0 .00) . The numbers of VSMCs in experimental groups were fewer than positive control group (P<0 .05) ,whereas the difference of OD value still has no statistical significance between experimental and positive groups (P= 0 .173) .Conclusion Recombinant Slit2 could inhibits migration in VSMCs induced by TNF‐α,whereas it has no effect on proliferation of VSMCs .关键词
肿瘤坏死因子α/Slit2蛋白/血管平滑肌/细胞增殖/细胞运动Key words
tumor necrosis tactor-alpha/Slit2 protein/muscle ,smooth ,vascular/cell proliferation/cell movement分类
医药卫生引用本文复制引用
刘丽华,刘涛,王浩宇,陈桂秀,倪伟,邓学云..Slit2蛋白对TNF-α诱导的大鼠胸主动脉血管平滑肌细胞增殖迁移的影响[J].重庆医学,2015,(4):462-464,3.
