重庆医学Issue(5)：605-607,3.DOI:10.3969/j.issn.1671-8348.2015.05.009
米诺环素对PC12细胞缺氧缺糖损伤后神经突生长的影响
Effects of minocycline on neurite outgrowth of PC12 cells following oxygen glucose deprivation
摘要
Abstract
Objective To investigate the effects of minocycline on cell viability and neurite outgrowth of pheochromocytoma cells (PC12) after oxygen‐glucose deprivation(OGD) injury .Methods PC12 cells were exposed to OGD insult for 2 ,4 ,6 ,8 h to estab‐lish a cerebral ischemia model in vitro .High‐differentiated PC12 cells were cultivated and randomly divided into three groups :con‐trol group ,OGD group and various doses of minocycline(0 .1 ,1 .0 ,10 .0 μM) treated group .24 h after OGD‐reperfusion ,PC12 cells viability was assessed by CCK‐8 assay ,the neurite was labeled with MAP‐2 by immunofluorescence and neurite length was meas‐ured by the Image‐Pro Plus 7 .0 software ,GAP‐43 protein expression was determined by Western blotting .Results Compared to the OGD groups ,minocycline induced a concentration‐dependent increase in cells viability [(46 .1 ± 2 .9)% vs .(77 .0 ± 2 .5)% ,P<0.01],improvedneuriteoutgrowthandincreasedtheexpressionofGAP‐43proteininPC12cellsafterOGDinjury([(0.34±0.04) vs .(2 .11 ± 0 .10) ,P<0 .01] .Conclusion Minocycline could protect against oxygen glucose deprivation injury and promote neurite outgrowth .This finding suggests minocycline may be a novel therapy for cerebral ischemia .关键词
米诺环素/神经突生长/氧糖剥夺/PC12细胞Key words
neurite outgrowth/minocycline/oxygen glucose deprivation/PC12 cell分类
医药卫生引用本文复制引用
陶涛,秦新月,冯金洲,罗华,李小刚..米诺环素对PC12细胞缺氧缺糖损伤后神经突生长的影响[J].重庆医学,2015,(5):605-607,3.基金项目
四川省卫生计生委资助项目（140032）；泸州市科技计划项目（2014S4506）；泸医附院青年基金（博士）资助项目（14046）。 （）
