重庆医学Issue(7)：873-875,3.DOI:10.3969/j.issn.1671-8348.2015.07.003
楤木皂苷通过降低 Akt／NF-κB 通路抑制HeLa 细胞的增殖并促进其凋亡
Araloside inhibited proliferation and promotes apoptosis of HeLa cells via suppression of Akt/NF-κB pathway
摘要
Abstract
Objective To explore the effect of araloside on the proliferation and apoptosis of HeLa cells.Methods Human cervical cancer cell line HeLa was cultured in vitro,the experiment was divided into 4 groups as follows:blank group,araloside trea-ted groups(50,100,200 μg/mL).Normal saline and araloside (50,100,200 μg/mL)were gave,respectively.24,48 and 72 hours lat-er,the cells were collected.Cell proliferation was detected by MTT,cell apoptosis was determined by flow cytometry,the expression of pAkt1,pIкBα,NF-κB (p65),Bcl-2 and Caspase-3 were evaluated by western blot.Results Araloside obviously inhibited the pro-liferation and increased the apoptosis level of HeLa cells in a time-dose dependent manner.Moreover,Araloside significantly de-creased the phosphorylation of Akt1 and IκBα,reduced the expression of NF-κB(p65)and Bcl-2,whereas obviously increased Caspase-3 content.Conclusion Araloside could inhibit the proliferation and promote the apoptosis of HeLa cells via suppressing Akt/NF-кB signaling pathway.关键词
楤木皂苷/HeLa 细胞/Akt/NF-κB/增殖/凋亡Key words
araloside/HeLa cells/Akt/NF-кB/proliferation/apoptosis分类
医药卫生引用本文复制引用
段海霞,李东红,暴蕾,李欢,高红敏..楤木皂苷通过降低 Akt／NF-κB 通路抑制HeLa 细胞的增殖并促进其凋亡[J].重庆医学,2015,(7):873-875,3.基金项目
陕西省科学技术研究发展计划基金资助项目（2013K12-03-22）。 （）
