重庆医学Issue(10)：1297-1299,1304,4.DOI:10.3969/j.issn.1671-8348.2015.10.001
腺病毒 rhBMP-2转染兔 BMSCs 复合同种异体脱钙骨基质的生物相容性研究
Study on biocompatibility of Ad-rhBMP-2 transfection on rabbit BMSCs combined with allogeneic DBM
摘要
Abstract
Objective To observe the biocompatibility of rabbit bone marrow mesenchymal stem cells (BMSCs)combined with allogeneic decalcified bone matrix(DBM)after transfecting adenoviral recombinant human bone morphogenetic protein-2(Ad-rhBMP-2).Methods The rabbit allogeneic DBM material was prepared according to the Ursit method.After transfecting Ad-BMP-2 on rabbit bone marrow mesenchymal stem cells,the immunohistochemical was used to detect the expression of BMP-2 in the transfected cells;after 48 h of transfection,the cells were planted on the allograft DBM,then the scanning electron microscopy was used to observe the cell growth and adhesion condition on material,and the proliferation condition of BMSCs was detected by MTT. Results After 48 h of adenoviral transfection,BMSCs could express BMP-2 successfully.The scanning electron microscopy showed that the cells after transfection adhered well and massively proliferated on DBM material.The MTT assay showed that the prolifer-ation condition of the cells after transfection planted on DBM was normal,which showed no statistically significant difference when compared with the control group (P >0.05).Conclusion The Ad-BMP-2 transfection on BMSCs is well biocompatible to allogene-ic DBM.关键词
骨形态发生蛋白质类/腺病毒科/骨髓/间质干细胞/脱钙骨基质Key words
bone morphogenetic proteins/adenoviridae/bone marrow/mesenchymal stem cells/decalcified bone matrix分类
医药卫生引用本文复制引用
蔡伟良,李强,宁寅宽,武成聪,陈佳滨,石正松..腺病毒 rhBMP-2转染兔 BMSCs 复合同种异体脱钙骨基质的生物相容性研究[J].重庆医学,2015,(10):1297-1299,1304,4.基金项目
国家自然科学基金资助项目（31160199）。 （）
