重庆医学Issue(11)：1516-1518,3.DOI:10.3969/j.issn.1671-8348.2015.11.026
基因芯片快速鉴定分枝杆菌菌种方法的建立
Establishment of a rapid and accurate gene microarray for identifying Mycobacterium species
摘要
Abstract
Objective To establish a rapid and accurate method for the identification of Mycobacterium species by the gene microarray and to verify its clinical application value .Methods According to the gene sequence of 23 species of Mycobacteria ,the specific probes were designed and the gene chips were prepared .23 Mycobacterial standard strains ,9 non‐mycobacterial strains ,103 clinically isolated mycobacterial strains were detected by PCR‐based reverse blot hybridization assay in the gene chip .Results 23 mycobacterial standard strains ,9 non‐mycobacterial strains were detected by gene chip ,the results showed that the specificity was 100% .Of 103 mycobacterial clinically isolated strains ,87 strains were identified as Mycobacterium tuberculosis compounds (MTC) and 16 strains as non‐tuberculosis mycobacteria (NTM ) including 5 strains of M .abscessus ,3 strains of M .intracellulare ,3 strains of M .avium ,2 strains of M .fortuitum ,1 strain of M .kansas ,1 strain of M .marinum and 1 strain of M .gordonae .The identification results of 103 clinically isolated strains were completely consistent with the sequencing results .The lowest detection limit by this method was 103 copies/mL .Conclusion The gene microarray technique for rapidly identifying Mycobacteria and differentiate MTC and NTM has the advantages of simpleness ,rapidness ,high accuracy ,high specificity and high sensitivity .关键词
分枝杆菌属/菌种鉴定/寡核苷酸序列分析Key words
mycobacterium/species identification/oligonucleotide array sequence analysis分类
医药卫生引用本文复制引用
唐曙明,李爱敏,陈海霞,杨自华..基因芯片快速鉴定分枝杆菌菌种方法的建立[J].重庆医学,2015,(11):1516-1518,3.基金项目
深圳市科技局资助项目（201102151）。 （）
