东南大学学报（自然科学版）Issue(3)：616-620,5.DOI:10.3969/j.issn.1001-0505.2014.03.029
气管滴注纳米二氧化钛对大鼠的亚急性肺毒性实验研究
Experimental study on subacute pulmonary toxicity induced by intra-tracheal instillation of nano-TiO2 in rats
摘要
Abstract
Sprague Dawley rats were exposed to intra-tracheal instillation of nano-TiO2 with different doses.The exposure was conducted twice a week,for four consecutive weeks.After four weeks, the subacute pulmonary toxicity was assessed by the hematological parameters,enzyme activities,C-reactive protein (CRP ) level, pathological examination and transmission electron microscopy (TEM).Results show that the neutrophil count (NEUT)increased in the group of 32 mg/kg nano-TiO2 and the content of hydrogen peroxide (CAT),glutathione (GSH)and superoxide dismutase (SOD)activities decreased.The results of lung histology demonstrate the expansion of lung gaps and slight alveolar thickness,increased proliferation of tissue macrophages of deposition massive par-ticulate,increased lymphocytes and fibrocyte with the increase of nano TiO2 dose.Type II cells have prominent lamellar bodies and the damage of lamellar bodies are observed by TEM.Nano-TiO2 can induce the change of enzyme activities and histology,and present inflammation and massive particu-late deposition in pulmonary tissues and pulmonary injury.关键词
纳米二氧化钛/肺毒性/氧化应激/亚急性毒性Key words
nano-TiO2/pulmonary injury/oxidative stress/subacute toxicity分类
医药卫生引用本文复制引用
常旭红,陈璐斯,杨天,唐萌,王蓓..气管滴注纳米二氧化钛对大鼠的亚急性肺毒性实验研究[J].东南大学学报（自然科学版）,2014,(3):616-620,5.基金项目
国家重大科学研究计划资助项目（2011CB933404）、中央高校基本科研业务费专项资金资助项目（CXZZ12-0122）． （）
