吉林医药学院学报Issue(5)：324-326,3.
JC-1单染法检测CCCP对内皮细胞线粒体膜电位的影响
Effect of CCCP on endothelial cells mitochondrial membrane potential by JC-1 single staining detection
摘要
Abstract
Objective Analyze the influence of carbonyl cyano-to-chlorobenzene hydrazone ( CCCP ) on human um-bilical vein endothelial cells ECV-304 mitochondria .Methods ECV-304 cell was cultured in vitro and treated with different concentrations of CCCP for 20 minutes .After straining by fluorescent probes Rh 123 or JC-1 ,cells were meas-ured by flow cytometry .Results The intensity of green fluorescence emitted by JC-1 monomer increased with the in-creasing of CCCP concentration .Conclusion The changing of cell MMP could be well detected by flow cytometry staining by new fluorescent probe JC-1 .关键词
线粒体膜电位/碳酰氰基-对-氯苯腙/流式细胞术Key words
mitochondrial membrane potential/CCPC/flow cytometry分类
医药卫生引用本文复制引用
官福新,周露露,张宸豪,李妍..JC-1单染法检测CCCP对内皮细胞线粒体膜电位的影响[J].吉林医药学院学报,2014,(5):324-326,3.基金项目
吉林省教育科学规划课题（GH11249）；吉林医药学院高等教育教学研究重点资助项目（2012-2014）；吉林省科技厅中青年科技创新领军人才及团队项目（20130521018JH）． （）
