吉林医药学院学报Issue(1)：12-14,3.
氯化钴诱导SH-SY5 Y细胞缺氧损伤作用的研究
Cobalt chloride induced hypoxia on SH-SY5Y cells
摘要
Abstract
Objective To investigate the hypoxia injury and mechanism of Cobalt chloride ( CoCl2 ) on SH-SY5Y cells.Methods Different doses of CoCl2 were used to treat SH-SY5Y cells,MTT assay was employed to examine the growth of the cells .ELISA was used to detect the expressions of Bax ,Bcl-2 ,Caspase-3 and HIF-1α.Results The in-hibitory effect of CoCl 2 was appeared in a time and dosage dependent manner .CoCl 2 could increase the expression of Caspase-3, Bax and HIF-1α(P<0.05),while reduce the expression of Bcl-2 (P<0.01).Conclusion CoCl2 could induce hypoxia on SH-SY5 Y cells by up-regulating HIF-1αand inducing apoptosis .关键词
CoCl2/SH-SY5Y细胞/缺氧模型Key words
CoCl2/SH-SY5Y cells/hypoxia model分类
医药卫生引用本文复制引用
邹荣军,梁祖凤,石婉婷,朱悦诚,李秋凤,郭馨,田晶..氯化钴诱导SH-SY5 Y细胞缺氧损伤作用的研究[J].吉林医药学院学报,2015,(1):12-14,3.基金项目
吉林省教育厅项目（2014369）；吉林省大学生创新创业训练项目（2013845）． （）
