动物医学进展Issue(5)：44-49,6.
猪化脓隐秘杆菌的分离鉴定及病原特性研究
Isolation and Identification of Arcanobacterium Pyogenes of Swine and Studies on the Pathogeny Speciality
摘要
Abstract
A strain of bacteria was isolated from the organs of a pig with pericarditis and pneumonia which came from a herd in Huizhou of Guangdong province.This isolation was identified as Corynebacterium pyogenes (Arcanobacterium pyogenes )preliminary after the analysis of morphological and biochemical. The 16 S rRNA genes and virulence genes as PLO,nanH,nanP,cbpA,fimA,fimG,fimC were amplified by PCR and sequenced.The results of Blast indicated that the nucleotide sequence homology of 16 S rRNA gene was 91.6%~95.0% between this isolation and other animals as wild boar,cattle,sheep,Lin She and so on.This confirmed that the isolation was Arcanobacterium pyogenes (A.pyogenes).The virulence genes as PLO,nanH,fimA,were detected and the nanP,cbpA,fimC,fimG genes were not.In Animal pathogenicity test,3 weeks old Babl/c mice were infected with the bacteria which the final concentration was 6.0×109 cfu/mL,then died after 24 h,indicated that this strain was more pathogenicity.Drug sensi-tive test indicated that this strain was high sensitive in cefazolin,cefradine,cefotaxime,novobiocin,ampicil-lin,vancomycin,andmoderate sensitive in doxycycline,enrofloxacin,amikacin,ofloxacin,and resistant in streptomycin,sulfamethoxazole,tetracycline,gentamicin,clindamycin,sulfamethoxazole,norfloxacin.This research provides the reference of identification,epidemiological investigation and control of porcine Ar-canobacterium pyogenes.关键词
猪/化脓隐秘杆菌/分离鉴定/病原Key words
Swine/Arcanobacterium pyogenes/isolation and identification/pathogeny分类
农业科技引用本文复制引用
张乐宜,蔡汝健,宋长绪..猪化脓隐秘杆菌的分离鉴定及病原特性研究[J].动物医学进展,2014,(5):44-49,6.基金项目
广东省科技厅项目（2012A020200014和2010A040301010）；广州市科技计划项目（12C14071615）；广东省生猪产业体系项目（粤农2009-380）；广东省农业科学院院长基金项目 （）
