动物医学进展Issue(5)：85-89,5.
两种不同土鳖虫肠道菌群总 DNA 提取方法的比较及 ERIC-PCR 指纹图谱分析
Comparison of Two Methods for Total DNA Extraction and Analysis of Intestinal Microflora in Eupolyphaga sinesis by ERIC-PCR Fingerprint
苏双良 1王慧 1李洪萍 2白秀娟1
作者信息
- 1. 东北农业大学动物科学技术学院，黑龙江哈尔滨 150030
- 2. 吉林农业大学中药材学院，吉林长春 130118
- 折叠
摘要
Abstract
To screen a method that could provide a high quality DNA template and investigate the similarity and diversity of intestinal microflora in Eupolyphaga sinesis,TIANamp Stool DNA Kit method and CTAB method were used to extract total DNA from intestinal microflora of the Eupolyphaga sinesis.The DNA quality was compared based on the concentration,purity and 16 S DNA PCR products,and analysis of intestinal microflora was carried out by ERIC-PCR fingerprint.The results showed that total DNA ex-tracted by each of the two methods could be used to 16 S DNA amplification and ERIC-PCR fingerprint a-nalysis.The quality of total DNA extracted by the two methods was good enough to study molecular ecol-ogy of intestinal microflora.But the result of ERIC-PCR of DNA extracted by CTAB method showed more microflora diversities.关键词
土鳖虫/肠道菌群/总DNA 提取/ERIC-PCRKey words
Eupolyphaga sinesis/intestinal microflora/total DNA extraction/ERIC-PCR分类
农业科技引用本文复制引用
苏双良,王慧,李洪萍,白秀娟..两种不同土鳖虫肠道菌群总 DNA 提取方法的比较及 ERIC-PCR 指纹图谱分析[J].动物医学进展,2014,(5):85-89,5.
