动物医学进展Issue(6)：16-20,5.
驽巴贝斯虫二温式 PCR 检测方法的建立及初步应用
Establishment and Application of Two-temperature PCR for Babesia caballi Detection
摘要
Abstract
To establish a rapid,specific and sensitive two-temperature PCR assay for detection of Babesia caballi ,specific primers were designed according to Bc-48 gene of Babesia caballi published in GenBank.A gene fragment of 155 bp was amplified by this method,and there was no cross reaction with B.bigemina, T.annulata,T.equi,T.sergenti,and the lowest detection limit of Babesia caballi DNA was 5.17 × 10 2 copies/μL.Eighty-two blood samples collected from horses in Hejing were detected by the established method.The results showed that the positive rate was 69.51%(57/82),while that of blood smear test was 37.8%(31/82).This study indicated that established two-temperature PCR method could be used for early diagnosis of B.caballi infection with its high specificity and sensitivity.关键词
马驽巴贝斯虫/Bc-48 基因/二温式PCR/样品检测Key words
Babesia caballi/Bc-48 gene/two-temperature PCR/sample detection分类
农业科技引用本文复制引用
张杨,达伊力特,刘进,李佳,巴音查汗..驽巴贝斯虫二温式 PCR 检测方法的建立及初步应用[J].动物医学进展,2014,(6):16-20,5.基金项目
国家科技支撑计划项目 （）
