动物医学进展Issue(6)：21-25,5.
猪衣原体实时荧光定量 PCR 检测方法的建立及应用
Development and Application of TaqMan-MGB Real-time PCR for Detection of C .cuis
摘要
Abstract
In order to establish quantitative PCR for detecting Chlamydia suis ,based on the MOMP gene of C .cuis,this study selected specific primers and probe from highly conserved region,the PCR reaction was developed by building the recombinant plasmid.The results showed that the detection limit of the assay was 1.7×102 copies/μL,the calibration curve equation:Y=-3.214 7x+41.218,a correlation coefficient of R2 = 0.999 1,expanding efficiency rate 104.6%.The method used to detect Chlamydophila psittaci , Chlamydophila abortus ,Chlamydia trachomatis ,Chlamydophila pneumoniae ,Chlamydophila pecorum , Chlamydia muridarum had no cross reaction.The real-time PCR was reproducible,the test of its intra-and inter-coefficients of variation from 0.495% to 1.752%.Compared with conventional PCR method,the detection rate of the method established in this study is higher.This method is important for detecting C . cuis and epidemiological investigation.关键词
猪衣原体/实时荧光定量 PCR/TaqMan-MGBKey words
C .cuis/real-time PCR/TaqMan-MGB分类
农业科技引用本文复制引用
袁曾壮,王昱,聂福平,杨俊,李应国,肖进文,王国民,李贤良,刘力..猪衣原体实时荧光定量 PCR 检测方法的建立及应用[J].动物医学进展,2014,(6):21-25,5.基金项目
质检公益性项目（201310093）；国家质检总局科技项目（2012IK028）；重庆市科委科技创新能力建设项目 （）
