动物医学进展Issue(6)：36-39,4.
牛病毒性腹泻病毒 NS3蛋白的原核表达及鉴定
Prokaryotic Expression and Identification of the NS3 Protein of Bovine Viral Diarrhea Virus
摘要
Abstract
This study aimed to provide pure NS3 protein as antigen for ELISA,which could distinguish BVDV vaccinated from infected cattle.A pair of primers were designed according to the major antigenic re-gion of bovine virus diarrhea NS3 gene derived form GenBank.The NS3 gene with a size of 1 206 bp was amplified by PCR and subcloned in to prokaryotic vector pET-32a.The recombinant plasmid pET-32a-NS3 was transformed in to E.coli Transetta(DE3)and then expressed by IPTG induction.The NS3 recombi-nant protein was analyzed by SDS-PAGE and Western blot.The result showed that the NS3 recombinant protein was mainly expressed in the form of inclusion body and the molecular weight of the fusion protein was about 61 ku.The result of the Western blot showed that the NS3 recombinant protein could be specif-ically recognized by positive serum to BVDV,and may be used as the coating antigen to develop a diagnosis kit against BVDV.关键词
牛病毒性腹泻病毒/NS3/原核表达/鉴定Key words
Bovine viral diarrhea virus/NS3/prokaryotic expression/identification分类
农业科技引用本文复制引用
范晴,谢芝勋,谢志勤,刘加波,庞耀珊,邓显文,谢丽基,罗思思..牛病毒性腹泻病毒 NS3蛋白的原核表达及鉴定[J].动物医学进展,2014,(6):36-39,4.基金项目
广西自然科学基金面上项目（2012GXNSFAA053074）；广西特聘专家专项 （）
