动物医学进展Issue(8)：40-43,4.
猪瘟病毒酵母双杂交 pGBK T7-NS3诱饵载体的构建与鉴定
Construction and Identification of CSFV pGBKT7-NS3 Bait Vector in Yeast Two-hybrid System
摘要
Abstract
The NS3 gene of classical swine fever virus was amplified by RT-PCR and confirmed by sequen-cing and enzyme digestion .The NS3 gene was then subcloned into pGBKT7 to construct the bait vector ac-cording to the reading frame .The reconstructed bait vector pGBKT7-NS3 was transformed into Y2H Gold yeast strain ,its self-activation and toxic effect were tested by the phenotype assay .The expression of pG-BKT7-NS3 was analysed by Western blot .As a result ,the successfully reconstructed bait vector pGBKT7-NS3 would not self-activitate the reporter gene and had no toxic effect to the yeast ,which laid the founda-tion for using yeast two-hybrid assay to screen interaction proteins .关键词
猪瘟病毒/NS3蛋白/酵母双杂交系统/诱饵载体Key words
Classical swine fever virus/NS3 protein/yeast two-hybrid system/bait vector分类
农业科技引用本文复制引用
宋江伟,田宏,吴锦艳,陈妍,尚佑军,刘湘涛..猪瘟病毒酵母双杂交 pGBK T7-NS3诱饵载体的构建与鉴定[J].动物医学进展,2014,(8):40-43,4.基金项目
国家现代农业产业技术体系项目 （）
