动物医学进展Issue(8)：44-48,49,6.
牛支原体分离株全基因组序列测定及初步分析
Complete Genome Sequence and Preliminary Analysis of Mycoplasma bovis Isolated Strain
王艳杰 1李平安 1吴太平 2关平原1
作者信息
- 1. 内蒙古农业大学兽医学院农业部动物临床诊疗技术重点实验室，内蒙古呼和浩特010018
- 2. 内蒙古农业大学食品科学与工程学院，内蒙古呼和浩特 010018
- 折叠
摘要
Abstract
The purpose of this test was to understand the genetic sequence of Mycoplasma bovis NM2012 strain ,and study its potential pathogenesis and its regulation mechanisms of replication ,transcription and translation .NM2012 strain in vitro and its genomic DNA was extracted by phenol-chloroform extraction method and sent to the Beijing Genomics Institute for whole genome sequence .The result showed that the genome size of Mycoplasma bovis NM2012 strain is 993 483 bp ,GC content is 29 .26% ,a total of three scaffolds and 24 contigs .Genome analysis found that Mycoplasma bovis NM2012 genome contains 885 genes ,total length is 834 042 bp ,average length is 942 bp ,representing 83 .95% of the full length ge-nome .Tandem repeats is 79 ,total length is 9 203 bp ,accounting for 0 .926 3% of full-length genome . There are 53 small satellite sequences ,5 microsatellite sequences ,34 tRNA and 4 rRNA .By constructing phylogenetic tree with 16 S rRNA sequence from sequenced Mycoplasma bovis NM2012 strain and Myco-plasma which mainly infecting cattle and sheep ,we further confirmed that NM2012 strain is Mycoplasma bovis .The study provides basis for further research on Mycoplasma bovis .关键词
牛支原体/酚-氯仿提取法/全基因组测序Key words
Mycoplasma bovis/phenol-chloroform extraction method/whole genome sequencing分类
农业科技引用本文复制引用
王艳杰,李平安,吴太平,关平原..牛支原体分离株全基因组序列测定及初步分析[J].动物医学进展,2014,(8):44-48,49,6.
