动物医学进展Issue(8)：65-69,5.
两株野鸭源新城疫病毒分离株F基因和HN基因的分子特性分析
Molecular Characterization of F Gene and HN Gene of Two Newcastle Disease Virus Isolates from Wild Ducks
摘要
Abstract
In order to analyze the molecular characteristics of F and HN genes of Newcastle disease virus (NDV) ,isolated from wild ducks in Changsha ,Hunan province .The F gene and HN gene of two NDV ,i-solated from feces of wild ducks ,were amplified by RT-PCR and genetically analyzed .The nucleotide length of F gene coding region is 1 662 bp ,coding 553 amino acids .The motif of cleavage site of the fusion protein was 112 E-R-Q-E-R-L117 ,which keeps consistent with the characteristcs of the reference Class I avir-ulent strains . Homology analysis of F gene revealed that sequence similarities with Class Ⅰ reference strains varied from 92 .3%-93 .8% .The HN gene contains a 1 851 bp open reading frame ,coding 616 ami-no acids .Homology analysis of HN gene showed 91 .6%-93 .9% of identities between the two isolates and Class Ⅰ reference strains .Phylogenetic analysis of the F gene and HN gene sequences revealed that the two isolates NDV are grouped into genotype 3 in Class Ⅰ .关键词
新城疫病毒/F基因/HN基因Key words
Newcastle disease virus/F gene/HN gene分类
农业科技引用本文复制引用
徐钰,张晓田,孙贺廷,初冬,高玉伟,解林红,耿海东,高晓龙,王海军,范胜涛,李元果,王铁成,吴长江..两株野鸭源新城疫病毒分离株F基因和HN基因的分子特性分析[J].动物医学进展,2014,(8):65-69,5.基金项目
国家科技支撑计划项目 （）
