动物医学进展Issue(9)：25-28,4.
高致病性猪繁殖与呼吸综合征病毒N基因真核表达载体的构建及表达
Construction of Eukaryotic Expression Vector and Expression of N Gene of HP-PRRSV
摘要
Abstract
In order to study the N protein structure,function and immunological characteristics of porcine reproductive and respiratory syndrome virus,the A06 RNA was extracted from PRRSV of Yunnan strain. The specific primers were designed to amplify the full-length N gene,372 bp fragment was cloned into ex-pression vector pFastBac HTB of baculovirus Bac-To-Bac,and further homologous recombination in E.coli DH10Bac.The eukaryotic expression vector containing N gene was successfully constructed.Transfection of Sf9 cells,the recovery of recombinant baculovirus were made.SDS-PAGE and Western blot assay indi-cated that recombinant N protein was expressed successfully with molecular weight of 17 ku which was consistent with the expected size and could specifically react with anti PRRSV positive sera.关键词
猪繁殖与呼吸综合征病毒/N基因/杆状病毒/表达Key words
Porcine reproductive and respiratory syndrome virus(PRRSV)/N gene/baculovims/expression分类
农业科技引用本文复制引用
廖立珊,吕建强,秦智锋,林庆燕,花群义,曾少灵,孙洁,杨俊兴,阮周曦,张彩虹,曹琛福,唐金明,叶奕优..高致病性猪繁殖与呼吸综合征病毒N基因真核表达载体的构建及表达[J].动物医学进展,2014,(9):25-28,4.基金项目
国家质检总局项目 （）
