动物医学进展Issue(9)：34-38,5.
猪葡萄球菌脱落毒素Exh C基因的克隆及原核表达
Cloning and Prokaryotic Expression of Exfoliative Toxin ExhC Gene of Staphylococcus hyicus
摘要
Abstract
This research was intended to study the molecular characteristics and reactogenicity of prokary-otic expression products of exfoliative toxin Exh C gene in S.hyicus.The Exh C gene was amplified in GDZC strain of S.hyicus which came from Zengcheng of Guangdong province by PCR.Sequence analysis indicated that the coding region has 837 bp,to code 279 amino acid.The nucleotide homology and amino acid homology were 100% both with Exh C gene of S.hyicus and S.sciuri which came from abroad.The recombinant expression plasmid pET-28a (+)-Exh C was shifted to E.coli BL21(DE3)for inducible ex-pression.After the SDS-PAGE,fusion protein was as inclusion body,and its molecular mass was 32 ku as the expected.Western blot indicated that recombinant protein of Exh C can response specifically with posi-tive serum of GDZC strain,and indicated that it has good immunologic activity.关键词
猪葡萄球菌/Exh C基因/原核表达Key words
Staphylococcus hyicus/Exh C gene/prokaryotic expression分类
生物科学引用本文复制引用
张乐宜,吴坚文,蔡汝健,李艳,蒋智勇,刘燕玲,宋长绪..猪葡萄球菌脱落毒素Exh C基因的克隆及原核表达[J].动物医学进展,2014,(9):34-38,5.基金项目
广东省科技厅项目（2010A040301010，2012A020200014，2012A061100006）；2011广东省主体科研机构创新能力建设专项（项目资助编号2011）；广州市科技计划项目（2012J4100064）；广东省生猪产业体系项目（粤农2009-380）及广东省农业科学院院长基金项目（201313）；科技部农业科技成果转化资金项目2013GB2E000352 （）
