动物医学进展Issue(9)：46-51,6.
绿原酸对小鼠不同组织巨噬细胞增殖、分泌及吞噬功能的影响
Effects of Chlorogenic Acid on Proliferation,Secretion and Phagoeytosis Functions of Different Tissue Phagocytes in Mice
摘要
Abstract
In order to investigate effects of chlorogenic acid (CHA)on mice macrophages of different tis-sues,mouse peritoneal,bone marrow and spleen macrophages by aseptic preparation were cultured in vitro and stimulated with lipopolysaccharide (LPS)in RPMI1640 medium containing CHA.MTT assay,ELISA and Griess methods were used to analyze cell proliferation,cell secretion levels of TNF-α and NO,and phagocytic activity of macrophages was detected by Wright-Giemsa staining.The results showed that there were significant decreases in A values and NO secretion levels of peritoneal,spleen and bone marrow mac-rophages in CHA groups and CHA+LPS groups compared with CHA groups and CHA+LPS groups,and there was a dose-dependent increase.Compared with LPS groups,the levels of TNF-αsecreted from mac-rophages of different tissues showed a decrease.There was a significant increase in TNF-α secretion of spleen and bone marrow macrophages in LPS+CHA groups,and not significant in that in peritoneal mac-rophages.CHA significantly enhanced phagocytic capacity of LPS-stimulated peritoneal macrophages.In addition,CHA significantly improved the survival of spleen and bone marrow macrophages from mouse treated with cyclophosphamide (CY),and there was a significant reduction in the amount of NO secreted from spleen and peritoneal macrophages.The results indicated that there were some degree effects of CHA on different tissue macrophage proliferation,TNF-α and NO secretion,and phagocytic activity of the mouse.关键词
绿原酸/巨噬细胞/增殖/分泌/吞噬/小鼠Key words
chlorogenic acid/macrophage/proliferation/secretion/phagoeytosis/mouse分类
农业科技引用本文复制引用
张永红,官佳懿,崔德凤,谷崇高,徐双,李洋,沈红..绿原酸对小鼠不同组织巨噬细胞增殖、分泌及吞噬功能的影响[J].动物医学进展,2014,(9):46-51,6.基金项目
北京市教委资助项目（KM201410020005）；2014北京农学院科技创新团队科研能力提升工程项目（KCT2014012）；国家科技计划课题 （）
