动物医学进展Issue(9)：54-57,4.
新型血红蛋白抗菌肽抑菌活性及溶血性研究
Studies on Antibacterial Activity and Hemolysis of Novel Hemoglobin Antimicrobial Peptide
摘要
Abstract
To analyze the bioactivity of novel hemoglobin antimicrobial peptide,the minimal inhibitory con-centration of hemoglobin antimicrobial peptide to Escherichia coli ,Staphylococcus aureus,Monilia albican was tested by broth microdilution method.The hemolysis was tested by measuring the release of hemoglo-bin.Thermal stability of hemoglobin antimicrobial peptide was determined by agar gel diffusion method. And the morphology of bacteria tesed were observed by scanning electron microscope.The results showed that the MIC to Escherichia coli ,Staphylococcus aureus ,Monilia albican were 0.5,1.0,0.25 mg/mL. It caused 6.7% rupture of erythrocyte at the concentration of 2.0 mg/mL.After heated,it kept stability partly.The shrinking,distorting and gathering of microorganism were observed.It indicated that hemo-globin antimicrobial peptide had a good bio-safety in the development and application,which had a signifi-cant antibacterial activity and low hemolysis.关键词
血红蛋白抗菌肽/抑菌活性/溶血性/热稳定性Key words
hemoglobin antimicrobial peptide/antibacterial activity/hemolysis/thermal stability分类
农业科技引用本文复制引用
张庆华,王青,尚田田,赵志雨,胡建和,徐彦召..新型血红蛋白抗菌肽抑菌活性及溶血性研究[J].动物医学进展,2014,(9):54-57,4.基金项目
国家自然科学基金项目 （）
