动物医学进展Issue(9)：70-73,4.
虎源传染性鼻气管炎病毒gD基因重组真核表达载体的构建
Construction of Eukaryotic Recombinant Expression Plasmid Containing gD Gene of Feline Herpesvirus Type 1 from Tiger
张晓明 1胡俊 1彭仕明 2陈武2
作者信息
- 1. 华南农业大学兽医学院，广东广州 510642
- 2. 广州动物园，广东广州 510070
- 折叠
摘要
Abstract
The primers of gD genes of the FHV-1 were designed according to sequences of the FHV-1 from GenBank,gD gene was amplified by PCR and cloned successfully into pMD18-T Simple vector.gD gene was constructed into eukaryotic expression vector pcDNA 3.1(+),the recombinant plasmid ,named as pcDNA-gD,was transfected into 293T cells.The results of RT-PCR and Western blot indicated that the gD gene was transcripted and expressed,and the recombinant fusion protein was about 42.29 ku.The results lay an important foundation for the further research of FHV-1.关键词
虎源传染性鼻气管炎病毒/gD基因/聚合酶链反应/真核表达Key words
Feline herpesvirus type 1/gD gene/PCR/eukaryotic expression分类
农业科技引用本文复制引用
张晓明,胡俊,彭仕明,陈武..虎源传染性鼻气管炎病毒gD基因重组真核表达载体的构建[J].动物医学进展,2014,(9):70-73,4.
