动物医学进展Issue(9)：85-89,5.
牛奶中青霉素G及青霉噻唑酸的UPLC-MS／MS法测定
Simultaneous Detection of Penicillin G and Benzylpenicillin Acid in Milk by UPLC-MS/MS
刘静 1邹明 2郑增忍 1赵思俊 3曲志娜 3王君玮 3孙晓亮 3谭维泉 3刘坤 3曹旭敏3
作者信息
- 1. 青岛农业大学，山东青岛 266109
- 2. 中国动物卫生与流行病学中心，山东青岛 266032
- 3. 中国动物卫生与流行病学中心，山东青岛 266032
- 折叠
摘要
Abstract
An ultra performance liquid chromatography tandem mass spectrometric (UPLC-MS/MS)anal-ysis method was established for simultaneous analysis of penicillin G and benzylpenicillin acid in milk.The milk samples were precipitated with acetonitrile:alcohol(3 ∶ 1,V∶ V),and the extracts were applied to HLB cartridge for clean up,the separation was performed by ultra performance liquid chromatography-tan-dem mass spectrometry.The correlation coefficients were above 0.993.The detection limits (S/N>3)of penicillin acid G and benzylpenicillin acid were 0.3 μg/kg-0.5 μg/kg.The detection limits (S/N>10)of penicillin G and benzylpenicillin were 1.0 μg/kg-2.0 μg/kg.The results quantified by matrix spiked cali-bration curve,the recoveries ranged from 75.88%-106.0%,and RSD were in the range of 6.65%-14.17%. The method is sensitive and repeatable,and can be applied to the field of residue analysis about penicillin and their metabolites.关键词
超高效液相色谱-串联质谱/青霉素G/青霉噻唑酸/牛奶Key words
UPLC-MS/MS/penicillin G/benzylpenicillin acid/milk分类
农业科技引用本文复制引用
刘静,邹明,郑增忍,赵思俊,曲志娜,王君玮,孙晓亮,谭维泉,刘坤,曹旭敏..牛奶中青霉素G及青霉噻唑酸的UPLC-MS／MS法测定[J].动物医学进展,2014,(9):85-89,5.
