动物医学进展Issue(9)：127-131,5.
黄喉拟水龟腐皮病病原菌的分离鉴定及药敏试验
Isolation,Identification and Antimicrobial Sensitivity Test of Bacterial Pathogens in Turtle Mauremys mutica with Skin-rotting Disease
兰云 1胡秀彩 1沈晓静 1李雪 1吕爱军 1朱爱华 1冯照军1
作者信息
- 1. 江苏师范大学生命科学学院，江苏徐州 221116
- 折叠
摘要
Abstract
To isolate and identify the pathogenic bacteria in turtle Mauremys mutica with skin-rotting dis-ease,and analyse the pathogenicity and drug sensitivity of the bacteria,the bacterial pathogens were isola-ted from the skin lesions of diseased Mauremys mutica ,and then identified by physiological and biochemi-cal characteristics,16 S rDNA sequencing,construction and analysis of phylogenetic tree;Furthermore, the drug sensitivity test and animal infection test were carried out by using standard methods.A novel Gram-negative,short rod-shaped bacterium,strain TSM13032,was isolated from the skin lesions of dis-eased turtle,and a 1 482 bp 16 S rDNA fragment of the isolate was obtained (GenBank accession number KF611897),the sequence homology of 16 S rDNA was 99.92% with Myroides sp.(GenBank accession number JX966100)from humans,the phylogenetic tree analysis showed that TSM13032 strain was clus-tered closely with newly reported bacterial isolate Myroides sp.;The pathogenicity to baby turtles was as-sessed by animal infection test.The antimicrobial sensitivity analysis showed that the isolate was highly sensitive to ampicillin,piperacillin,cefoperazone,cephalothin,cefamandole,azithromycin,nalidixic acid, chloramphenicol and sulfisoxazole.A newly isolated strain was identified as Myroides sp.,this is the first report isolated Myroides sp.from diseased Mauremys mutica .关键词
黄喉拟水龟/类香味菌/分离鉴定/致病性/药敏试验Key words
Mauremys mutica/Myroides/isolation and identification/pathogenicity/antimicrobial sensitivity test分类
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兰云,胡秀彩,沈晓静,李雪,吕爱军,朱爱华,冯照军..黄喉拟水龟腐皮病病原菌的分离鉴定及药敏试验[J].动物医学进展,2014,(9):127-131,5.
