动物医学进展Issue(10)：7-9,3.
一株口蹄疫病毒非结构蛋白3A 单克隆抗体的表位肽段鉴定
Identification of Peptide Covering Epitopes Corresponding to A Monoclonal Antibody Against Non-structural Protein 3A from Foot-and-mouth Disease Virus
摘要
Abstract
In order to identify the peptide region covering epitopes recognized by a monoclonal antibody 3A24# a-gainst non-structural protein 3A from foot-and-mouth disease virus,the protein 3A was prokaryotically expressed in two peptides,3A1 and 3A2,covering 1-89 and 77-153 amino acids.Primers targeting the genes 3a1 and 3a2 were designed and synthesized,respectively.Then the genes 3A1 and 3A2 were amplified by PCR and cloned into plas-mid pET-30a(+)-PES-SUMO.The recombinant plasmids were transformed into E.coli BL21(DE3)pLys and in-duced for expression by IPTG.The expressed peptides were examined by Western blot using monoclonal antibody 3A24# to identify the epitopes covering peptides.SDS-PAGE analysis showed that both 3A1 and 3A2 peptides were successfully expressed with the respective molecular weights as 26 ku and 25 ku.Western blot analysis showed that,both 3A1 and 3A2 can be recognized by FMDV infected cattle serum,but only 3A2 can react with monoclonal antibody 3A24 #.The epitopes recognized by monoclonal antibody 3A24 # are located on peptide 3A2,namely between the amino acids 89-153 of FMDV non-structural protein 3A.关键词
口蹄疫病毒/单克隆抗体/抗原表位Key words
Foot-and-mouth disease virus/monoclonal antibody/antigen epitope分类
农业科技引用本文复制引用
王娜,卢曾军,曹轶梅,李平花,付元芳,孙普,曾巧英,刘在新..一株口蹄疫病毒非结构蛋白3A 单克隆抗体的表位肽段鉴定[J].动物医学进展,2014,(10):7-9,3.基金项目
甘肃农业大学伏羲杰出人才项目；“十二五”国家高技术研究发展计划（863计划）项目 （）
