动物医学进展Issue(10)：30-33,34,5.
布鲁菌二氢硫辛酰胺脱氢酶的原核表达
Prokaryotic Expression of Brucella Dihydrolipoamide Dehydrogenase
摘要
Abstract
The dihydrolipoamide dehydrogenase(BMEI0145)gene was cloned from Brucella melitensis ,and its prokaryotic expressing vector was constructed,the antigenicity of BMEI0145 protein was analyzed.The full length of BMEI 0145 gene was amplified from Brucella melitensis 043 genome.The amplified fragment was cloned into a pMD18-T vector,the gene fregment after sequencing was digested with double enzymes and cloned into pET-28a vector.The recombinant expressing vector was transformed into E.coli BL21 (DE3)competent cells.After induction with IPTG,the expressed products were identified by SDS-PAGE and Western blot.The results suggested that we successfully cloned BMEI0145 gene of 1 404 bp length. The results of SDS-PAGE and Western blot showed that BMEI0145 protein with 62 ku specificly reacted with Brucella positive serum.Collectively,our data showed that BMEI 0145 protein was successfully ex-pressed and had the same antigenicity with the proteins of Brucella,which laid the foundation for further investigating Brucella genetic engineering vaccines and diagnosing brucellosis.foundation.关键词
布鲁菌/二氢硫辛酞胺脱氢酶/克隆/原核表达Key words
Brucella/dihydrolipoamide dehydrogenase/clone/prokaryotic expression分类
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关团,张辉,王震,孙志华,刘娟,韩玉霞,王文华,江雅丽,陈创夫..布鲁菌二氢硫辛酰胺脱氢酶的原核表达[J].动物医学进展,2014,(10):30-33,34,5.基金项目
国家自然科学基金项目 （）
