动物医学进展Issue(11)：6-9,4.
小反刍兽疫病毒H蛋白的原核表达和免疫学活性鉴定
Prokaryotic Expression and Immunoreactivity Identification of Peste des Petits Ruminants Virus Haemagglutinin Protein
摘要
Abstract
In order to study the biological activity of hemagglutinin protein of peste des petits ruminants vi-rus (PPRV)and supply materials for establishing immunological assay for detection of antibodies against PPRV,the PPRV H gene was amplified by RT-PCR using the specific primers designed according to the H gene sequence of PPRV published in GenBank.The amplified gene was cloned into a prokaryotic expres-sion vector pET52/LIC to obtain a prokaryotic expression vector pET52/LIC-PPRV H.Then the recombi-nant pET52/LIC-PPRV H was transformed into E.coli BL21 (DE3)for protein expression induced by IPTG.The expressed protein was tested by SDS-PAGE and Western blot,and result showed that the ex-pressed PPRV H protein was approximately 75 ku and could reacted with the positive sera against PPRV. The expressed PPRV H protein could be used as an antigen for PPRV antibody detection.关键词
小反刍兽疫病毒/H蛋白/原核表达Key words
Peste des petits ruminants virus (PPRV)/H gene/prokaryotic expression分类
农业科技引用本文复制引用
陶虹,杨俊兴,孙洁,李健波,刘建利,阮周曦,张彩虹,曹琛福,吕建强,花群义..小反刍兽疫病毒H蛋白的原核表达和免疫学活性鉴定[J].动物医学进展,2014,(11):6-9,4.基金项目
国家863计划项目（2012AA101301）；国家科技支撑计划项目 （）
